Before investigating the cytotoxic effect of the combination treatment with the EGFR inhibitor cetuximab and the Akt1/2/3 inhibitor MK2206, we determined the expression of Akt isoforms in HNSCC patients using RNA sequencing data from the TCGA dataset (Provisional, RNASeqV2 RSEM, 522 sequenced HNSCC patients). This evidence concerns the gene AKT1 and head and neck squamous cell carcinoma.