The results showed that rescue of TRIM50 significantly restored the decreased cell proliferation (Supplementary Figure 2B) and invasion (Supplementary Figure 2C), and reversed EMT (Supplementary Figure 2D) caused by TRIM50 depletion in PANC-1 and SW1990 cells, further confirming the requirement of TRIM50 in EMT-associated phenotypes of pancreatic cancer. Here, TRIM50 is linked to familial pancreatic carcinoma.