To further verify whether histamine played a tumor-promoting effect through estrogen receptors, AZD9496 (an ERα inhibitor), PHPPT (an ERβ inhibitor), PPT (an ERα agonist), and DNP (an ERβ agonist) were used to subject OVCAR-3 cells to the desired treatment (Figure 3D). The gene discussed is ESR1; the disease is neoplasm.