In the current study, in order to evaluate the potential of anti-GNA11 autoantibody in the diagnosis of ESCC, the level of anti-GNA11 autoantibody in sera of ESCC patients and matched normal controls was detected by ELISA, and the protein level and mRNA level of GNA11 were further explored by immunohistochemistry (IHC) and bioinformatics. The gene discussed is GNA11; the disease is esophageal squamous cell carcinoma.