BCL2 and adrenal cortex carcinoma: To investigate whether the ACC cells underwent apoptosis in the treatment of Sal, flow cytometry was performed to detect the apoptosis rate, WB was used to detect the expression of the apoptosis-related protein Bcl-2, and JC-1 staining was used to detect changes in the in-mitochondrial membrane potential of the cells to confirm the occurrence of apoptosis.