Previous studies also demonstrated that electroacupuncture could alleviate brain ischemia injury and contribute to neuroprotection against cerebral ischemia mainly by interacting with the NF-kappaB signaling pathway (35), PI3K/Akt/p53 pathway (36), and SIRT1-FOXO1 signaling pathway (37), which were in accordance with our RNA-seq analysis results. The gene discussed is AKT1; the disease is Cerebral ischemia.