Overall, key miRNA-mRNA networks were identified based on RNA-seq and RT-qPCR validations, and we found that miR-425-5p-Cdk1, mmu-miR-1186b-Prc1, mmu-miR-434-3p- Prc1, and mmu-miR-453-Prc1 were crucial networks regulated by EA in brain neuronal cells of ischemic stroke. Here, PRC1 is linked to ischemic stroke.