As shown in Figures 6A–F, CTCF overexpression counteracted the effect of gadenoside on the expression levels of NLRP3, GSDMD, Caspase-1 p20, IL-1β, CTCF, and DPP4 in the NAFLD model. This evidence concerns the gene NLRP3 and metabolic dysfunction-associated steatotic liver disease.