In the current study, we evaluated lipid profile markers including levels of TC and TG and liver disease markers AST and ALT (Sookoian and Pirola, 2015) in serum collected from the NAFLD animal model and found a significant reduction of the above markers in the presence of either gardenoside, si-CTCF, or si-DPP4, accompanied by attenuated hepatic steatosis in an animal model and reduced lipid droplets accumulation in cell model as revealed by oil Red O and H&E staining, respectively. This evidence concerns the gene CTCF and fatty liver disease.