To explore the hepatoprotective mechanism of gardenoside and its influence on the regulation of inflammasome and pyroptosis in NAFLD, we preliminarily examined the influence of gradient concentrations of gardenoside on lipid accumulation and the expression of proteins involved in the stimulation of NLRP3 inflammasome and caspase-1-induced pyroptosis. Here, CASP1 is linked to metabolic dysfunction-associated steatotic liver disease.