Since many studies (Wang et al., 2014; Fan et al., 2017) have confirmed the relationship between NAFLD and the PI3K/AKT/NF-κB pathway, NF-κBp65, the critical target of the PI3K/AKT/NF-κB signaling pathway, was selected to validate our findings. Here, NFKB1 is linked to metabolic dysfunction-associated steatotic liver disease.