CHAT and Alzheimer disease: The NSC line overexpressing the human choline acetyltransferase (ChAT) gene recovers the learning/memory deficits and elevates the levels of acetylcholine (ACh) in CSF when transplanted into rat brain of an experimentally induced AD model, in which the application of ethylcholine mustard aziridinium ion (AF64A) specifically inactivates the cholinergic nerves.