Wnt5a and Wnt11 were both increased from 3 days and become higher in a time-dependent manner compared with the sham group (Fig. 2B), and the fibrotic-related markers including Col-1, MMP2, MMP9, and TGF-β1 showed the similar expression profile at different time-points after TAC (Fig. S1C). The gene discussed is TGFB1; the disease is persistent truncus arteriosus.