Our findings suggest that an HFD can be used to create a mouse model of both hyperglycemia and obesity, and that prolonged imipramine intake in mice causes weight gain; increased food intake; increased kidney, liver, and fat pad weight; heightened serum and hepatic triglyceride, ALT, and AST levels; and increased fatty liver scores and adipocyte sizes. Here, GPT is linked to obesity due to melanocortin 4 receptor deficiency.