INS and obesity due to melanocortin 4 receptor deficiency: In addition, in order to confirm the inhibitory effect of BSE differentiation on adipocytes, 3T3-L1 pre-adipocytes were treated with DMI (a cocktail of dexamethasone, 3-isobutyl-1-methylxanthine, and insulin) to induce differentiation and treated with BSE and Garcinia cambogia extract, which is known to have anti-obesity effects [46].