Mice of both genotypes (WT littermate controls and TAM-treated Mttpf/f Villin Cre ERT2 mice) were fed an MCD diet for 3 weeks, revealing macrovesicular steatosis (Fig. 1A) with Mttp-IKO mice showing ∼80% reduction (1,700 → 385 μg/mg protein) in hepatic TG content as well as reduced cholesterol and FFA (Fig. 1B). This evidence concerns the gene MAPK3 and steatosis.