QRT-PCR was used to further validate the expression levels of 20 hub genes (B4GALT3, EDEM3, MTX1, STK17B, GGH, YBX1, ITM2A, COPA, LGALS1, DDX3Y, ITM2C, MAP3K14, TAPBPL, JUNB, CSGALNACT1, PLEK, NUCB2, PECAM1, ISCU and PPCDC) in the primary plasma cells from 20 patients with MM, including 15 patients without t(4;14) and 5 patients with t(4;14). Here, ISCU is linked to Miyoshi myopathy.