TNF and colorectal carcinoma: In a recent randomized, double-blind placebo study, it was concluded that the probiotic oral therapy by six viable strains (Lactobacillus and Bifidobacteria) for 6 months was demonstrated to significantly affect the expression of pro-inflammatory markers (IL-6, IL-10, IL-12, IL-17A, IL-17C, IL-22, and TNF-α) in CRC subjects, also within the study the treatment did not aggravate diarrheal frequency and canceled the necessity of antibiotic therapy [63].