TMPRSS13 and cancer: To investigate whether endogenously expressed TMPRSS13 is also N-linked glycosylated, we employed two different methods to assess its glycosylation in human cancer cells: (1) tunicamycin-mediated inhibition of N-linked glycosylation in live cells (58, 59), and (2) assessment of glycosylation status by PNGase F treatment of whole cancer cell lysates.