In addition, we detected EGFR expression in CMTM3 overexpressed JHC7 and U-CH1 cells and CMTM3 silenced MUG-Chor1 cells by Real-time PCR and found that CMTM3 did not alter EGFR expression at the mRNA level (Additional file 1A), indicating CMTM3 might facilitate EGFR degradation in chordoma cells. Here, EGFR is linked to chordoma.