CMTM3 and chordoma: Transwell assays were then performed to evaluate the invasion of CMTM3 in chordoma cells precoated with Matrigel in the chamber and we found that overexpressed CMTM3 remarkably inhibited invasion of JHC7 cells (Fig. 2I) and U-CH1 cells (Fig. 2J), and knockdown of CMTM3 revealed the opposite effects in MUG-Chor1 (Fig. 2K) and U-CH1 cells (Fig. 2L).