Indeed, when we analysed the ischaemic brain of WT and IRG1−/− MCAO mice, we found increased CD68+ MG and CD86+ MG as well as elevated Iba1+ cells in the ischaemic brain of IRG1−/− MCAO mice compared to WT MCAO mice, demonstrating IRG1 deficiency promotes MG activation in ischaemic stroke. Here, ACOD1 is linked to ischemic stroke.