To determine whether APA captures genes with clinical relevance, we performed a multivariate Cox regression correcting for patient age, sex, race, and tumor stage for each gene and identified a strong prognostic signature based on the APA usage pattern of 12 genes in lung cancer (Fig. 2f and Supplementary Data 8), including the type I transmembrane glycoprotein immunoglobulin CD96 and a key homologous recombination repair gene POLA2. Further, gene ontology analysis of APA events associated with survival revealed enrichment of cellular metabolic processes (Supplementary Data 9). This evidence concerns the gene POLA2 and neoplasm.