We found that the Q2 (Annexin V-FITC + PI+), Q3 (Annexin V-FITC + PI-) and Q2 + Q3 subpopulations of SHG-140 or T98G were all significantly increased after interference with ARF4 compared to the control group, which tentatively suggests that ARF4 is associated with anti-apoptotic in GBM. This evidence concerns the gene ANXA5 and glioblastoma.