TNF and infection: Tumors were allowed to engraft in the recipient lungs for 3 days before animals were treated with 2 × 106 BM cells ex vivo pre-loaded for 1 h with one of three test viruses: vMyx-M135 knockout (M135KO), vMyx-M11 knockout (M11KO), or vMyx-hTNF (MYXV expressing human TNF) (all at a multiplicity of infection [MOI] of 10) (Figure 1A).