GNRH1 and Follicular Cyst: The current study supported the new modified of GPG protocol, (GPGMH; Figure 2) by the inclusion of mifepristone (with a dose of 0.4 mg/kg along the second GnRH), AI after 24 h of mifepristone or second GnRH, and post-AI hCG (at day 5 of AI), is a choice regimen to enhance ovulation and pregnancy rates by reducing the incidence of follicular cysts and silent estrus in crossbred buffaloes during the breeding season.