Furthermore, through 1000 LASSO analyses, four hub genes (Figure 2C) were finally identified, namely: ankyrin repeat and sterile alpha motif domain containing 4B (ANKS4B), Cathepsin D (CTSD), tissue inhibitor of metalloproteinase 1 (TIMP1), and zinc finger protein 703 (ZNF703), which are the most stable prognostic-related genes associated with M2 macrophages in colon cancer. Here, CTSD is linked to malignant colon neoplasm.