Compared with a negative control, proliferation of prostate cancer cells was significantly reduced (P < 0.0001; t-test) (Fig. 4c; Supplementary Data 1) in response to MDM4 depletion using two distinct single-guide RNAs (sgRNAs) (Extended Data Fig. 9, Supplementary Data 2). The gene discussed is MDM4; the disease is prostate cancer.