The PI3K pathway levels were correlated with different kinases across cancer types, including AKT3 and PIK3CA in MB (R = 0.47, FDR = 0.02 and R = 0.55, FDR = 2.5e − 3, respectively), AKT3 and RPS6KB1 in PRAD (R = 0.35, FDR = 0.025 and R = 0.34, FDR = 0.04, respectively), and AKT1 and PIK3CA in STAD (R = 0.36, FDR = 0.02 and R = 0.33, FDR = 0.03, respectively). This evidence concerns the gene RPS6KB1 and gastric adenocarcinoma.