More specifically, we examined the vasculature of the MDA-MB-231-LM2 and AsPC-1 3D xenograft tumours via immunohistological staining of formalin-fixed paraffin-embedded tumours sections using an antibody to CD31 and PAS stain to define angiogenesis (CD31+PAS+) as well as VM structures (CD31−PAS+). This evidence concerns the gene PECAM1 and neoplasm.