Here, we assessed three different biomarkers of HRR deficiency: (i) tumor BRCA1/2 mutation status; (ii) presence of HRR gene mutations by targeted NGS (using our CLIA-certified institutional OncoPanel assay27); and (iii) presence of mutational signature 3 (Sig3), a specific mutational signature characterized by a high number of larger deletions (up to 50 bp) with overlapping microhomology at breakpoint junctions31. The gene discussed is BRCA1; the disease is neoplasm.