CASP3 and breast cancer: Subsequently, the effect of MTDH on cleaved caspase-3 in Gem-treatment breast cancer cells was further examined by Western blot, and we found that in Gem-treated MCF7 cells silencing MTDH increased the expression of cleaved caspase-3 (P < 0.01, P < 0.05, P < 0.001, Fig. 2C), which was partially reversed by overexpressed MTDH (P < 0.01, P < 0.05, P < 0.001, Fig. 2D).