GEM and breast carcinoma: Transwell was performed to detect the effect of MTDH on the invasion of Gem-treated breast cancer cells, and the data indicated that silencing MTDH inhibited the cell invasion but significantly promoted the inhibition of Gem-treated cell invasion (P < 0.01, P < 0.05, P < 0.001, Fig. 3A), whereas overexpression of MTDH enhanced cell invasion and significantly reduced cell invasion previously inhibited by Gem (P < 0.05, P < 0.001, Fig. 3B).