We transfected ZFAS1 siRNA, miR-497-5p mimic, miR-497-5p mimic plus the HMGA2 plasmid and ZFAS1 siRNA plus the miR-497-5p inhibitor into PC cells and measured HMGA2 protein levels in different groups via western blotting (Fig. 5A). This evidence concerns the gene HMGA2 and pachyonychia congenita.