HMGA2 and pachyonychia congenita: We detected the abundance of HMGA2 mRNA in Ago2/RNA induced silencing complex (RISC) after up-regulation of miR-497-5p, and found that the levels of HMGA2 and miR-497-5p were enriched in RISC in PC cells with miR-497-5p overexpression (Fig. 4C).