Consistent with what has been previously reported in other cancer cell lines, such as for breast, colorectal and pancreatic cancers [25–27], LIN28B overexpression in immortalised cholangiocyte and cholangiocarcinoma cell lines enhanced cell proliferation, migratory capacity and clonogenic potential, suggesting that LIN28B is capable of promoting cancer stem cell-like properties in transformed bile duct epithelium. Here, LIN28B is linked to cholangiocarcinoma.