In summary, our data show for the first time the role of MRTF-A as a key orchestrator in regulating TGF-β-induced PD-L1 transcription in NSCLC cells and reveal that p65 is a new executive transcription factor for MRTF-A in coactivating PD-L1 transcription. This evidence concerns the gene TGFB1 and non-small cell lung carcinoma.