Furthermore, we generated a double knockout mouse model (Pcyox1−/−/Apoe−/− mice) for studying the role of PCYOX1 in atherosclerosis, and PCYOX1 analysis by IHC and ISH was also performed on aortic root sections of Pcyox1+/+/Apoe−/− and Pcyox1−/−/Apoe−/− mice on high-fat diet (HFD) for 8 weeks. The gene discussed is APOE; the disease is atherosclerosis.