We previously determined the downstream effects of the ASXL1 mutation using the KBM5 chronic myelogenous leukemia (CML) cell line, that carries a homozygous nonsense ASXL1G710X mutation, resulting in loss of ASXL1 protein expression [12]. Here, ASXL1 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.