To investigate how NKD1 regulates β‐catenin expression, the HCT116 colon cancer cells were transfected transiently with pcDNA3 (empty plasmid) or pcDNA3‐NKD1 (plasmids expressing NKD1 proteins) for 48 h, the cells were then conducted with cycloheximide (CHX) for different time points to impede the protein translation. Here, NKD1 is linked to malignant colon neoplasm.