The present study revealed that Homer1 expression at the mRNA and protein levels was day-dependently upregulated in the auditory cortex of salicylate-treated mice, while Homer2 or Homer3 expression showed no significant alteration in response to salicylate, indicating that salicylate-induced tinnitus may result from downregulation of the Homer1 gene in the autitory cortex. This evidence concerns the gene HOMER3 and Tinnitus.