Subsequently, the protein level of ACTN1 was assessed by WB, which showed that 8 of the 10 pairs had increased levels of ACTN1 within GC samples relative to adjacent samples; this finding was further verified by RT-qPCR of 19 GC samples and matched non-carcinoma samples (P = 0.0042) (Figure 2a-b). Here, ACTN1 is linked to carcinoma.