CASP3 and necrotizing enterocolitis: Subsequently, the expression of Caspase-3 and Cleaved Caspase-3 in the ileum was detected using qRT-PCR and Western blotting, respectively, and celecoxib reduced Caspase-3 and Cleaved Caspase-3 expression in the ileum of NEC rats (all P < 0.05, Figure 5c-d).