Specifically, since SC preadipocytes from IR/T2D obese individuals showed diminished SF3B1 tv1 expression levels (Figure 3—figure supplements 1 and 2), we exposed SGBS cells to pladienolide-B, which binds to the SF3B complex and inhibits pre-mRNA splicing via targeting splicing factor SF3B1 (Aouida et al., 2016; Cretu et al., 2018). Here, SF3B1 is linked to type 2 diabetes mellitus.