PPARG and obesity due to melanocortin 4 receptor deficiency: PRPF8 expression recovery upon PRPF8-pcDNA3.1 transfection increased the expression of several PPARG isoforms (PPARG-2, PPARG-3, PPARG-4, and γORF4) to control levels (i.e. SC preadipocytes in NG obesity), especially in IR preadipocytes (Figure 4C).