PRPF8 and type 2 diabetes mellitus: First, we examined the effect of PRPF8 silencing in SC preadipocytes from obese NG patients using a specific siRNA as a mean to mimic the down-regulation of this gene found in SC preadipocytes from obese subjects with IR/T2D (Figure 2A and B, Figure 2—figure supplement 1 and 2).