Western blot analysis and RT-qPCR were used to test H3K27me3 abundance and FOXP4-AS1, EZH2, and ZC3H12D expression in human normal liver cells L-02 and human HCC cell lines MHCC-97H, HepG2, LM3, and SMMC-7721. Here, FOXP4 is linked to hepatocellular carcinoma.