In a triple comparison of one patient (YUHS 12), comprising of the primary tumor, an enucleated sample after brachytherapy, and a liver metastasis sample, when the latter samples were compared to the primary tumor, one gene (METRK) mutations in the enucleation sample and six gene (FANCA, KMT2D, PCLO, PGR, TET3, and ZBTB7A) mutations in the metastasis were found to be newly occurred, and were each exclusive without common mutations (Fig. 1B). Here, KMT2D is linked to digestive system neoplasm.