Therefore, our findings of DLGAP1-AS2 binding to Six3 to relieve its repression on Wnt1 transcription thus expanded our understanding of the role of DLGAP1-AS2 and Six3 in the regulation of tumor biology, and support the pursuit of DLGAP1-AS2/Six3/Wnt1/β-catenin as a potential prognostic indicator and therapeutic targets for GC. Here, DLGAP1 is linked to neoplasm.