PNPLA1 and ichthyosis: The ichthyosis mutation was initially identified in Golden Retrievers caused by an indel mutation in the PNPLA1 gene, although Embark also tests for variants associated with the NIPAL4 gene from American Bulldogs, the SLC27A4 gene initially found in Great Danes, and the FAM83H gene in Cavalier King Charles Spaniels [34–37].