The study highlights for the first time that β-blocker can improve the efficacy of tumor virotherapy in gastric cancer and preloaded β-blocker could enhance viral replication through promoting the secretion of IFNα and further upregulating the p-STAT3 and then inhibiting the induction of the IFN-responsive antiviral gene-PKR, even in the presence of type I IFNs. This evidence concerns the gene EIF2AK2 and gastric cancer.