The result showed that the levels of Ac-tubulin were apparently reduced in PCV2-, PCV2-Rep1- or PCV1-Cap2-infected cells with the increase of infection time, but not in PCV1-, PCV1-Rep2- or PCV2-Cap1-infected cells, indicated that the deacetylase activity of HDAC6 was markedly enhanced in the cells expression PCV2 Cap protein, despite all of them did not show a significant change in HDAC6 protein levels (Fig 7F). This evidence concerns the gene CHML and infection.