Further target gene prediction and gene ontology analysis demonstrated that the most relevant signal pathways for the Silva pattern A of EAC were PI3K-Akt-mTOR signaling pathways and focal adhesion, which is consistent with the clinicopathological characteristics of the Silva pattern A. It is well known that PI3K-Akt-mTOR signaling pathway regulates fundamental cellular functions such as transcription, proliferation and metabolism, component genes of which are commonly activated in cancer [25]. This evidence concerns the gene AKT1 and cancer.