KEGG signaling pathway analysis showed that the genes in cluster 0 were mainly enriched in protein digestion and absorption and TGF beta signaling pathway, which regulated the digestion and absorption of cell debris after apoptosis; The genes in cluster 3 are mainly enriched in the PI3K-Akt signaling pathway and pathways in cancer, which regulates cell apoptosis; The genes in Cluster 4 are mainly enriched in fluid shear stress and atherosclerosis, and hepatocellular carcinoma, which also plays a role in regulating apoptosis. The gene discussed is AKT1; the disease is cancer.