Aerobic glycolysis is an important hallmark that distinguishes cancer tissues from normal tissues; on the one hand, it interacts with oncogenes PI3K, HIF-1, and C-MYC for inducing chemoresistance by facilitating the overexpression of glucose transporters and key enzymes of glycolysis and resistant signaling pathways in different degrees, and on the other hand, it acts synergistically with hypoxia and acidosis for advocating oncogene signaling pathways and stromal cells on sustaining energy supply and immune escape in cancer. This evidence concerns the gene HIF1A and cancer.