Cui et al. (2021) utilized lentivirus in vitro infection and in vivo administration methods to prove that knockdown of ACSL4 alleviated brain injury after IS. Zhao et al. (2020) performed real-time polymerase chain reaction (PCR) to analyze the association between PNPLA2 rs1138693 (T > C) genotype and the risk of IS. Wang J.F. et al. (2020) proved P2RX2 as an up-regulated gene in myocardial infarction using gene ontology (GO) analysis and pathway enrichment analysis in a comparative study of gene expression profiles rooted in acute ischemia and infarction. The gene discussed is PNPLA2; the disease is myocardial infarction.