While investigating the effects of PRL/PRLR on the proliferation of 786O and ACHN cells treated with PBS control and 100 ng/mL prolactin for 24 h, CCK-8 assay and colony formation assay results suggested that PRL drastically promoted ccRCC cell proliferation (Figures 4A,B). Here, PRL is linked to nonpapillary renal cell carcinoma.