In detail, genetic deletion of Senp3 specifically in Treg cells led to the expression of T effector-related genes such as Ifng, Il4, Il13, Il17a, Il22, and Il9 and loss of Treg cell-specific genes, such as Foxp3 and Pdcd1. Senp3-induced Treg destabilization resulted in increased frequencies and effector function of CD4+ and CD8+ T effector cells infiltrating the tumors and reduction of tumor growth in MC38 colon carcinoma model and B16F10 melanoma model. Here, CD8A is linked to melanoma.